Biostratigraphic study and re-examination of 22 late Eocene to early Oligocene sections provides data in support of three and possibly a fourth late Eocene impact events in the G. index Zone during a period of about 1 Ma of the middle Priabonian between 34.7 and 35.7 Ma. No major species extinctions or significant species abundance changes directly coincide with these impact events. Species extinctions are gradual and selective, affecting primarily cool-temperature-intolerant surface dwellers. These extinctions began with the onset of global cooling during the early middle Eocene and culminated near the Eocene/Oligocene boundary. The global cooling was associated with the isolation of Antarctica as Australia moved northward, the development of a circum-Antarctic circulation, growth of Antarctic ice sheet beginning by late middle Eocene time and a change from a thermospheric to thermohaline circulation. We find no evidence that the multiple late Eocene impact events directly contributed to the climatic deterioration already in progress.  PDF